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Epithelioma papulosum cyprini

WebAbstract. Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, … WebMar 14, 2024 · Epithelioma Papulosum Cyprini (EPC) cells were maintained at 24°C in GMEM/HEPES 25 mM medium supplemented with 2 mM L-glutamine (PAA) and with 10% fetal bovine serum (FBS) (Eurobio) . rVHSV were propagated in monolayer cultures of EPC cells at 15°C as previously described . Virus titers were determined by plaque assays on …

CRISPR/Cas9-mediated knockout of HIF-1α gene in …

WebEPC [Epithelioma Papulosum Cyprini] is an epithelial cell line that was isolated in 1969 from the skin of a fathead minnow. This cell line was deposited by J Winton. Product category Animal cells Organism Pimephales promelas, Fathead minnow Cell type … WebMar 1, 2001 · Biology. Biotechnology Letters. The variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) … the velvet willow tulsa https://slk-tour.com

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WebWhen cell monolayers of epithelioma papulosum cyprini (EPC), fathead minnow (FHM), chinook salmon embryo (CHSE-214), and bluegill fry (BF2) were pretreated with 7% PEG-20,000, they produced 4-17-fold increases in plaque assay titers of IHNV. The plaque assay titers of viral hemorrhagic septicemia virus, chum salmon reovirus, and chinook salmon ... WebApr 1, 2024 · LMBV demonstrates strong infectivity in epithelioma papulosum cyprini (EPC) cells and can cause the cytopathic effect (CPE) in this cell line. EPC cells were cultured in Medium 199/EBSS (M199, HyClone) supplemented with 10% fetal bovine serum (FBS, HyClone) containing streptomycin (100 μg/mL) and penicillin (100 μg/mL) at 25 °C. WebApr 1, 1983 · A cell line, named Epithelioma papulosum cyprini (EPC) and originating from carp epidermal herpes virus-induced hyperplastic lesions, was esta blished and … the velvet waynesboro pa

eCFR :: 50 CFR 16.13 -- Importation of live or dead fish, mollusks, …

Category:CRISPR/Cas9-mediated knockout of HIF-1α gene in epithelioma papulosum ...

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Epithelioma papulosum cyprini

Frontiers Structural and Functional Analyses of Type I IFNa Shed ...

WebOct 5, 2015 · In this study, synthetic siRNA molecules were designed to target SVCV-N and SVCV-P transcripts to inhibit SVCV replication and were tested in an epithelioma papulosum cyprini (EPC) cell line.

Epithelioma papulosum cyprini

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WebMar 22, 2024 · Furthermore, mutation of Leu27, Glu103, Lys117, and His165 markedly decreased the phosphorylation of signal transducer and activator of transcription (STAT) 1a induced by CiIFNa in the Epithelioma papulosum cyprini (EPC) cells, and Glu103 was shown to be required for the CiIFNa-activated antiviral activity. WebFeb 15, 2024 · To investigate the effect of CPF exposure on epithelioma papulosum cyprini (EPC) cell pyroptosis and the role of the miR-124-3p/CAPN1 axis, we …

WebApr 13, 2024 · 线粒体提取试剂盒用于从动物细胞或组织中分离出完整而纯化的线粒体。. 适合于动物软组织（肝或脑组织）和硬组织（肌肉）以及培养细胞的线粒体的制备。. 其制备物产量高，可以被用于细胞凋亡、信号传递、代谢和蛋白组学等的研究。. 索莱宝的线粒体提取 ... WebTransfection of fish cells Epithelioma papulosum cyprini (EPC) cells (Fijan Results et al. 1983) were grown in wells of 24-well plates at 28 C with 400 µl of Dutch modified RPMI, 20 mM Several transfection …

WebMay 14, 2007 · Interestingly, the epithelioma papulosum Cyprini cells derived from Cyprinus carpio are restrictive to the virus. These results indicate that CyHV-3 is not restricted to common carp and koi, but other cyprinids are also vulnerable to the virus. Download to read the full article text References WebCurrent lineages of the epithelioma papulosum cyprini(EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells J Winton, J Winton US Geological Survey, Western Fisheries Research Center, Seattle, WA, USA Search for more papers by this author W Batts, W Batts

WebMar 1, 2001 · The variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) with the genes for -galactosidase from E.coli, for luciferases from...

Webepithelioma papulosum cyprini cells (EPC) and rainbow trout gonad cells (RTG-2) • Designed primers and cloned several genes from the IHNV genome into expression vectors such as pcDNA3.1(-) using ... the velveteen cabinetWebOct 28, 2024 · Epithelioma papulosum cyprini (EPC) cells were grown in Leibovitz medium (L-15, Sigma) enriched with 10% fetal bovine serum (FBS, Welgene) and penicillin-streptomycin (Welgene). Interferon regulatory factor 9 (IRF9) gene knockout EPC (ΔIRF9-EPC) cells established in our previous study [ 22] were also cultured under the same … the velveteen dream\u0027s arrest videoWebSep 17, 2024 · Citation 16 Moreover, in vitro study confirmed the role of chlorpyrifos in triggering epithelioma papulosum cyprini cell pyroptosis via miR-124-3p/CAPN1 axis. Citation 67. Conclusion. Similar to that in mammals, pyroptosis in fish is also activated by caspases through NLRP3 inflammasome to cleave GSDME and release its NT domain, … the velvet wormWeb丁香通为您找到7条dna认祖科学吗厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关dna认祖科学吗产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购dna认祖科学吗，上丁香通。 the velveteen dress ff14WebDexamethasone treatment decreases replication of viral hemorrhagic septicemia virus in Epithelioma papulosum cyprini cells [J]. Kim Min Sun, Lee Su Jin, Choi Seung Hyuk, Archives of virology . 2024,第5 期. 机译：地塞米松治疗降低了上皮细胞瘤中胞质瘤中的病毒出血性败血症病毒的复制 ... the velvet witchWebThe variables involved in the transfection of epithelioma papulosum cyprini (EPC) cells (a representative carp fish cell line) with the genes for β-galactosidase from E. coli, for … the velveteen in stillwaterWebVolume 33, Issue 8p. 701-704 Current lineages of the epithelioma papulosum cyprini(EPC) cell line are contaminated with fathead minnow, Pimephales promelas, … the velveteen rabbit 1922 book first edition