Webbead-based protocol allows micro-scale purification of up to 15 µg of 6xHis-tagged protein per well.If larger amounts of purified protein are needed, a vacuum-controlled Ni-NTA resin-based process provides a convenient medium-scale method for purification of up to 300 µg of 6xHis-tagged protein in a 96-well format.This protocol has been adapted Web80 µg or as much as 400 mg of GST fusion protein using Glutathione Sepharose 4B (27-4574-01). Yield of fusion protein is highly variable and is affected by the nature of the fusion protein, the host cell, and the culture conditions used. Fusion protein yields can range from 1-3 mg/l up to 10 mg/l (2). Table 1 can be
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WebMagnetic beads for protein purification Magnetic beads are used to purify single proteins, large protein complexes, antibodies and for high-throughput purifications. Magnetic beads based on chromatography resins provide functions of the resin with the convenience and ease-of-use of magnetic beads. WebGlutathione S-transferase (GST) pull-down assays revealed an interaction of the PDZ domain of SNX27 with AQP2c. ... Interactions between recombinant SNX27 proteins and beads … bouncy keyboard symbols
Automated High-Throughput Purification of 6xHis-Tagged …
WebThe high binding capacity base stable AmMag™ magnetic beads used in conjunction with our ergonomic (patent pending) AmMag™ magnetic racks allow the fast and efficient … WebCentrifuge the samples again at 750 g for 1 minute at 4°C to pellet the beads. Remove the supernatant. The fusion protein can be stored on the beads at 4°C at this stage. This is appropriate if the protein is to be labeled or used in a GST pull-down experiment. 19. Add 5 ml of ice-cold PBS with protease inhibitors. bouncy kingdom