Phenylacetate-coa
WebPhenylacetyl-CoA is often produced via the reduction of ATP to AMP and the conversion of phenylacetate and CoA to diphosphate and Phenylacetyl-CoA. ATP + phenylacetate + CoA … WebDec 23, 2008 · The phl gene, encoding a PCL (phenylacetate–CoA ligase), was cloned in Escherichia coli as a maltose-binding protein fusion and the biochemical properties of the enzyme were characterized. The recombinant fusion protein converted PAA into phenylacetyl-CoA in an ATP- and magnesium-dependent reaction.
Phenylacetate-coa
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WebDownload alignment. phenylacetate-CoA oxygenase, PaaG subunit. Phenylacetate-CoA oxygenase is comprised of a five gene complex responsible for the hydroxylation of phenylacetate-CoA (PA-CoA) as the second catabolic step in phenylacetic acid (PA) degradation. Although the exact function of this enzyme has not been determined, it has … Web2-phenylacetate + ATP + CoA = AMP + diphosphate + phenylacetyl-CoA 1 publication This reaction proceeds in the forward direction. 1 publication EC:6.2.1.30 ( UniProtKB ENZYME Rhea) Source: Rhea 20956 Hide Rhea reaction 2-phenylacetate CHEBI:18401 zoom + ATP CHEBI:30616 zoom + CoA CHEBI:57287 zoom = AMP CHEBI:456215 zoom + diphosphate
Webphenylacetic acid: [ fen″il-ah-se´tik ] a catabolite of phenylalanine ; it is excessively formed and excreted, sometimes conjugated with glutamine , in phenylketonuria . WebJul 21, 2010 · Phenylacetyl-CoA is the substrate of a presumed multicomponent oxygenase, PaaABCDE. This oxygenase is a key enzyme of the pathway, proposed to be responsible for the introduction of oxygen into the aromatic ring of phenylacetyl-CoA ( 6, 14, 21 ). First we … We would like to show you a description here but the site won’t allow us.
WebApr 21, 2006 · The acyl-CoA ligand is bound at the protomer-protomer interface with the acyl-thioester moieties inserted into the core of the dimer. The ligand CoA units rest on … WebApr 21, 2006 · Phenylacetate derives primarily from phenylalanine, but it is also formed in degradation pathways that target a variety of environmental aromatics of natural and synthetic origin and, thus, is the central element of an important catabolon ( 20, 21, 22 ).
WebApr 15, 2008 · The phenylacetate-CoA ligase gene ( paaK) was cloned and heterologously expressed in Escherichia coli and the recombinant protein was purified. The enzyme catalyzed phenylacetate + CoA + MgATP → phenylacetyl-CoA + AMP + MgPP i with a V max of 24 μmol/min/mg protein at a temperature optimum of 75°C.
WebApr 2, 2011 · Phenylacetate. Ind3, a phenylacetate CoA ligase-like protein, activates indolmycenic acid by conjugating to AMP and catalyzes amide bond formation between indolmycenic acid-AMP and the terminal amino group of 4-dehydroarginine, which is followed by a ring-closure reaction to give an intermediate with the characteristic … new leaf books wholesaleWebCatalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA). Involved in the phenylalanine metabolism. 2 publications. Catalytic activity 2-phenylacetate + ATP + … intl priorityとはWebDec 1, 2014 · In addition, transcription of candidate genes for the anaerobic phenylacetate degradation, benzoyl-CoA degradation and glutaryl-CoA degradation pathways was significantly upregulated in microarray and quantitative real-time-PCR studies comparing phenylacetate-grown cells with acetate-grown cells. These results suggested that the … new leaf book distributorsWebThe Azoarcus evansii gene which codes for phenylacetate-CoA ligase, an enzyme involved in the aerobic degradation of phenylacetate, was isolated from a genomic library, using as the probe a fragment of the gene which encodes the isoenzyme that is induced under anaerobic conditions. By this means both the gene and its flanking sequences were … intl pro /100s driverWebAug 10, 2010 · Although a phenylacetate metabolic gene cluster had been identified, the underlying biochemistry remained largely unknown. Here we elucidate the catabolic pathway functioning in 16% of all... new leaf breadWebApr 26, 2011 · Phenylacetate is first activated through the addition of CoA. The phenylacetyl-CoA is then converted to epoxide by a multicomponent monooxygenase. The aromatic … new leaf bonsaiWebPhenylacetate. Optimal Result: 0 - 0.18 mcg/mg creatinine. Interpret your laboratory results instantly with us. Phenylacetate is produced from bacterial degradation of unabsorbed … new leaf bookstore