2024 Photocleavable spacer

Photocleavable spacer

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August undefined, 2024

WebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would … WebPC Biotin Alkyne an azide-reactive photocleavable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin. This reagent contains a biotin moiety linked to an azide group through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 ...

Spacer Phosphoramidites - RNA / BOC Sciences

WebMar 19, 2003 · New cationic lipids having an o-nitrobenzyl moiety as a photocleavable spacer between its hydrophilic and hydrophobic region were synthesized. To improve the efficiency of transfection with lipoplexes, … hp smart adapter bypass

Photo-cleavable spacer - Integrated DNA …

WebJan 6, 2003 · This unique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphoramidite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry ( 8 ... WebJul 2, 2024 · We were able to identify photocleavable peptides that bound tightly to HLA-DR1 and generated the peptide-receptive conformation after UV exposure. However, slow release of photocleaved peptide fragments from the binding site limited the rate of binding of an incoming labeled peptide and complicated kinetic measurements of the individual steps ... WebThe light-triggered release of paclitaxel or fluorophore Cy5 was achieved by incorporation of photocleavable spacers into the RNA nanoparticles. Upon irradiation with ultraviolet light, cargos were rapidly released (within 5 min). In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed ... hp smart ac adapter message

Photo-cleavable spacer - Integrated DNA Technologies

WebFeb 5, 2010 · DNAzymes incorporating photocleavable spacers were switched “on” or “off” with light. A DNAzyme (left) with two photocleavable spacers, in a binding arm and the catalytic core, lost the ability to hydrolyze RNA upon UV photolysis. Conversely, a circular DNAzyme (right) attached to a self-complementary strand via two photocleavable ... WebAug 1, 1995 · While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm to a ... fghbbjWebThe Vertiflex Procedure is redefining the treatment of LSS for patients. It provides patients with a clinically proven, minimally invasive solution that is designed to deliver long-term relief from the leg and back pain associated … fghdz

"WebSpacer Modifiers. The hydrophilic spacers; Spacer 9 ( LK2128) and Spacer 18 (HEG) ( LK2129) are also particularly useful to build in flexibility within an oligo sequence. 6. As with 3’-‘spacers’ these are known to be nuclease … " - Photocleavable spacer

Photocleavable spacer

http://genelink.com/oligo_modifications_reference/OMR_generate_pdf.asp?mod_sp_cat_id=22 WebSep 17, 2024 · In vitro treatment of breast cancer cells with the RNA nanoparticles harboring photocleavable paclitaxel showed higher cytotoxicity as compared to RNA nanoparticles without the photocleavable spacer. The methodology provides proof of concept for the application of the light-triggered controlled release of drugs from RNA nanocages. …

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WebPhotocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon … WebConveniently introduce spacer modifications into your oligonucleotide during synthesis with our high-quality spacer phosphoramidites and solid supports. Adding a spacer adds …

WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any … Photo-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC Spacer is a 10-atom linker arm that can only be cleaved when exposed to UV light within the appropriate spectral range. The resulting oligo will have a 5’ phosphate group that is available for ...

WebPhotocleavable spacer modified oligonucleotides could be used to tether an antisense oligo to a dextran, which is too large to pass through the nuclear pores. Photocleavage would then be used to release the oligo, thereby decoupling antisense effects within the cytoplasm from antisense effects within the nucleus. WebThe PC spacer (LK2131) and PC linker (LK2066) amidites can be incorporated within an oligonucleotide sequence. This is particularly useful in adding a modifier as a phosphoramidite where there is a need to remove this from the oligonucleotide at a later stage. ... Photocleavable Peptide-Oligonucleotide Conjugates for Protein Kinase Assays …

WebAug 1, 1995 · This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any substrate. In experiments using [leucine]-enkephalin as a model substrate, we show that PCB retains its high affinity toward avidin/streptavidin and allows rapid (< 5 min) and …

WebPhotocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. 订购说明： 1、绝大部分产品备有现货，一般情况下都能订货当日发货。 2、部分非常用产品，需提前1－2日预订，海外期货则需要提前3-6周预订。 hp smart adapter popupWebStorage Conditions: -20°C To -70°C. Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free … fghdjkWebBio-Synthesis offers photocleavable PC Biotin Oligo Modification at various scales. This light activatable PC biotin modified oligonucleotide contained a photocleavable spacer between the biotin group and the DNA bases. The biotin group is separated from the 5' end nucleotide base by the photo-cleavable group and a long chain spacer arm. fghgbbWebPhotocleavable Azido-NHS ester reagent contains an amine reactive group linked to azido moiety through a spacer arm containing a photocleavable moiety. Molecules jointed through photocleavable linker can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm 2). fghfgyWebPhotocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW ... hp smart adapter keeps popping upWebIMAGING SPACERS. Imaging spacers are ultra-thin adhesive spacers which peel-and-stick to coverglass or microscope slides to confine specimens without compression. Layer … hp smart ac adapterWebPC Linker (photocleavable) Spacer C3. Spacer C12. Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens), and is used to incorporate a spacer arm into an oligonucleotide. Spacer 9 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 9 has been used to form non-nucleotide bridges ... hp smart adapter buy