WebbTotal ubiquitylated proteins were affinity -purified using TUBE2-agarose beads. Polyubiquitylated CKla proteins, pulled down with TUBE2 resin, were kept on ice as control (lane 1) or treated with or without recombinant USP15 (rUSP15) protein at 37°C for 0.5 h (lanes 2 & 3, respectively), followed by IB analysis. CKla(Ub)n: polyubiquitylated CKla. Webb4 dec. 2014 · A Different Look at CKLA In October of 2014, the AD HOC Common Core Implementation Committee of the Rochester City Schools, which is one of the 5 big city …
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