Tritc-phalloidin
WebAdd Phalloidin-Tetramethylrhodamine Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with TRITC filter Important Warm the vial to room temperature and centrifuge briefly before opening. Storage and Handling Conditions WebPhalloidin-TRITC C60H70N12O13S2 CID 74932833 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological ...
Tritc-phalloidin
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WebJul 3, 2024 · Human intestinal cell models are widely used to study host-enteric pathogen interactions, with different cell lines exhibiting specific characteristics and functions in the gut epithelium. In particular, the presence of mucus may play an important role in adhesion and invasion of pathogens. The aim … WebTRITC is an orange-red fluorescent probe，Phalloidin, a cyclic heptapeptide toxin derived from Amanita phalloides, selectively binds filamentous actin F-actin with high affinity (Kd= 20 nM), but not monomeric actin g-actin [1 2]. TRITC Phalloidin staining has strong specificity and high contrast.
WebMay 3, 2024 · Figure 2a,b shows RBL-2H3 cells before (a) and after (b) stimulation stained with phalloidin-TRITC, which specifically binds to actin filaments in cells, on the SPRI-IMP sensor chip. When RBL-2H3 cells were stimulated with DNP-HSA (50 ng/mL), morphological changes, such as ruffling and spreading, were observed even on the sensor chip with the ... WebPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals.
WebOct 1, 2006 · TRITC-phalloidin-labeled actin filaments (7-nM final concentration) were then added. To reduce photobleaching, the final buffer contained 100 mM β -mercaptoethanol and an oxygen-scavenger enzyme system ( 16 ). To access actin filaments with the AFM cantilever tip, the top coverslip was gently removed from the flow-through microchamber. … WebPhalloidin - TRITC. Catalog Number: orb1289367. Be the first to review this product. Datasheet; Select Product Size. 50 Assays. 300 Assays. Quantity-+ Usually dispatched …
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WebPhalloidin conjugated to TRITC via the side chain of amino acid 7. The conjugate has a molecular weight of 1300. This fluorochrome has an excitation maximum of 540 nm and … my nationwide online banking loginWebPhalloidin-TRITC is a fluorescein derivative of Phalloidin, which can specifically label myof lin and display red fluorescence when labeled and can be observed using Tesred channels. For research use only. We do not sell to patients. Custom Peptide Synthesis Phalloidin-TRITC Chemical Structure CAS No. : 915013-10-4 Get it March 28 by noon. my nationwide pet insurance loginWebPlace each coverslip in a glass petri dish and extract it with a solution of acetone at ≤–20°C or 0.1% Triton X-100 in PBS. Leave for ~ 5 minutes. 2X Wash cells with PBS. When staining, dilute 5 μL Phalloidin-FITC methanolic stock solution into 200 μL PBS for each coverslip. To reduce nonspecific background staining add 1% bovine serum ... my nationwide insurance accountWebTetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. old people shaky handsWebPhalloidin is a phallotoxin isolated from Amanita phalloides, the death cap mushroom. Fluorescent phallotoxins stain F-actin at nanomolar concentrations. They are water … old people shaking handsWebDescription The VECTASHIELD HardSet Antifade Mounting Medium with TRITC-Phalloidin prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Fluorescent … old people servicesWebTRITC-phalloidin (P1951) and p -phenylenediamine (P6001) are from Sigma-Aldrich (St. Louis, MO). C3 toxin (BML-G130) is from Enzo Life Science (Plymouth Meeting, PA) or can be purified from Escherichia coli transformed with a plasmid encoding a glutathione S-transferase/C3 toxin fusion protein ( Pierce et al., 1999 ). 1. my nationwide on line account log in